This documentation is for the older Mesmerize legacy desktop application. Please see the home page or GitHub repo for information on the latest packages. https://github.com/kushalkolar/MESmerize
Add a Sample to the Project¶
When you are happy with the ROIs in the viewer for the current CNMF(E) derived or manually created ROIs, you can add this as a Sample to your project.
Each sample in your project contains the following:
The imaging data from which ROIs were extracted (the video)
All the ROIs with their spatial location, temporal dynamics, and any tags that you have entered in the ROI Manager.
Stimulus mappings, if your project is configured for this.
Meta data (that were associated with the imaging video), the date, video framerate.
Any further information that you have chosen to add based on your Project Configuration
If your ROIs were obtained through CNMF/CNMFE the following attributes from the final cnm object are stored: cnm.A, cnm.b, cnm.C, cnm.f, cnm.YrA
To add the current viewer work environment (see above) as a sample to your project, go to File -> Add To Project. You will be presented with a window similar to this:
The entries that you are prompted with directly correspond to the custom columns in your Project Configuration.
Every Sample in a project has a unique SampleID which is the combination of AnimalID + TrialID.
You can never change the AnimalID or TrialID (i.e. SampleID) since these are partially used as unique identifiers. A workaround is described in the FAQ for Project Organization.
AnimalID and TrialID are separated by the
-_- character combination when stored as a SampleID. Therefore do not use that character combination within your AnimalID or TrialID.