#!/usr/bin/env python3
# -*- coding: utf-8 -*-
"""
Created on April 23 2018
@author: kushal
Chatzigeorgiou Group
Sars International Centre for Marine Molecular Biology
GNU GENERAL PUBLIC LICENSE Version 3, 29 June 2007
"""
from ..core.common import ViewerUtils
from .pytemplates.cnmf_pytemplate import *
from ...common import get_window_manager
from ...common.qdialogs import *
from ...common.utils import HdfTools
from uuid import UUID
from shutil import copy
import os
from copy import deepcopy
import logging
logger = logging.getLogger()
[docs]class ModuleGUI(QtWidgets.QDockWidget):
def __init__(self, parent, viewer_reference):
self.vi = ViewerUtils(viewer_reference)
QtWidgets.QDockWidget.__init__(self, parent)
self.ui = Ui_DockWidget()
self.ui.setupUi(self)
self.ui.btnAddToBatchCNMF.clicked.connect(lambda: self.add_to_batch())
[docs] @present_exceptions()
def get_params(self, *args, group_params: bool = False) -> dict:
"""
Get a dict of the set parameters.
If the work environment was loaded from a motion correction batch item it put the bord_px in the dict.
Doesn't use any arguments
:return: parameters dict
:rtype: dict
"""
if self.vi.viewer.workEnv.imgdata.meta['fps'] == 0:
raise KeyError('No framerate for current image sequence!',
'You must set a framerate for the current image sequence. '
'You can set it manually in the console like this:\nget_meta()["fps"] = <framerate>')
# Get width of border that is NaN, usually happens due ot motion correction
history_trace = self.vi.viewer.workEnv.history_trace
try:
bord_px = next(d for ix, d in enumerate(history_trace) if 'caiman_motion_correction' in d)['caiman_motion_correction']['bord_px']
except StopIteration:
bord_px = 0
rf = self.ui.spinBoxRf.value()
# CNMF kwargs
cnmf_kwargs = \
{
'p': self.ui.spinBoxP.value(),
'nb': self.ui.spinBoxnb.value(),
'merge_thresh': self.ui.doubleSpinBoxMergeThresh.value(),
'rf': rf if not self.ui.checkBoxRfNone.isChecked() else None,
'stride': self.ui.spinBoxStrideCNMF.value(),
'K': self.ui.spinBoxK.value(),
'gSig': [
self.ui.spinBox_gSig_x.value(),
self.ui.spinBox_gSig_y.value()
],
'ssub': self.ui.spinBox_ssub.value(),
'tsub': self.ui.spinBox_tsub.value(),
'method_init': self.ui.comboBox_method_init.currentText(),
'border_pix': bord_px,
}
# Any additional cnmf kwargs set in the text entry
if self.ui.groupBox_cnmf_kwargs.isChecked():
try:
_kwargs = self.ui.plainTextEdit_cnmf_kwargs.toPlainText()
cnmf_kwargs_add = eval(f"dict({_kwargs})")
cnmf_kwargs.update(cnmf_kwargs_add)
except:
raise ValueError("CNMF kwargs not formatted properly.")
# Component evaluation kwargs
eval_kwargs = \
{
'min_SNR': self.ui.doubleSpinBoxMinSNR.value(),
'rval_thr': self.ui.doubleSpinBoxRvalThr.value(),
'use_cnn': self.ui.checkBoxUseCNN.isChecked(),
'min_cnn_thr': self.ui.doubleSpinBoxCNNThr.value(),
'cnn_lowest': self.ui.doubleSpinBox_cnn_lowest.value(),
'decay_time': self.ui.spinBoxDecayTime.value(),
'fr': self.vi.viewer.workEnv.imgdata.meta['fps']
}
# Any additional eval kwargs set in the text entry
if self.ui.groupBox_eval_kwargs.isChecked():
try:
_kwargs = self.ui.plainTextEdit_eval_kwargs.toPlainText()
eval_kwargs_add = eval(f"dict{_kwargs}")
eval_kwargs.update(eval_kwargs_add)
except:
raise ValueError("Evaluation kwargs not formatted properly.")
if self.vi.viewer.workEnv.imgdata.ndim == 4:
is_3d = True
else:
is_3d = False
# Make the output dict
d = \
{
'item_name': self.ui.lineEdName.text(),
'refit': self.ui.checkBoxRefit.isChecked(),
'border_pix': bord_px,
'is_3d': is_3d
}
# Group the kwargs of the two parts seperately
if group_params:
d.update(
{
'cnmf_kwargs': cnmf_kwargs,
'eval_kwargs': eval_kwargs
}
)
# or not
else:
d.update(
{
**cnmf_kwargs,
**eval_kwargs
}
)
return d
[docs] @present_exceptions()
def add_to_batch(self, params: dict = None) -> UUID:
"""
Add a CNMF batch item with the currently set parameters and the current work environment.
"""
if self.vi.viewer.workEnv.isEmpty:
QtWidgets.QMessageBox.warning(self, 'Empty work environment', 'The work environment is empty, '
'nothing to add to batch')
return
input_workEnv = self.vi.viewer.workEnv
if params is None:
d = self.get_params(group_params=True)
else:
# Check that user passed dict is formatted correctly
required_keys = ['cnmf_kwargs', 'eval_kwargs', 'item_name', 'refit', 'border_pix']
if any([k not in params.keys() for k in required_keys]):
raise ValueError(f'Must pass a params dict with the following keys:\n'
f'{required_keys}\n'
f'Please see the docs for more information.')
d = deepcopy(params)
name = d['item_name']
self.vi.viewer.status_bar_label.showMessage('Please wait, adding CNMF: ' + name + ' to batch...')
if self.ui.groupBox_seed_components.isChecked():
seed_path = self.ui.lineEdit_seed_components_path.text()
if not os.path.isfile(seed_path):
raise FileNotFoundError(
"Seed file does not exist, check the path"
)
try:
seed_params = HdfTools.load_dict(seed_path, 'data/segment_params')
except:
seed_params = 'unknown'
d['use_seeds'] = True
d['seed_params'] = seed_params
else:
d['use_seeds'] = False
batch_manager = get_window_manager().get_batch_manager()
u = batch_manager.add_item(
module='CNMF',
name=name,
input_workEnv=input_workEnv,
input_params=d,
info=d
)
if u is None:
self.vi.viewer.status_bar_label.clearMessage()
return
if d['use_seeds']:
logger.info("Copying component seed file")
copy(seed_path, os.path.join(batch_manager.batch_path, f'{u}.ain'))
self.vi.viewer.status_bar_label.showMessage('Done adding CNMF: ' + name + ' to batch!')
self.ui.lineEdName.clear()
return u